A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases.
نویسندگان
چکیده
Derivatization of a 5'-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.
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ورودعنوان ژورنال:
- Chemical communications
دوره 52 98 شماره
صفحات -
تاریخ انتشار 2016